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March-April2003  
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Well oiled solutions

Extensive application of emerging technologies will help India make a qualitative leap in the oilseeds sector, elaborates DM Hegde

In India, the demand for both edible and non-edible oils is increasing with the increase in population and improvement in the standard of living. The oil consumption rate is increasing at six per cent and the per capita availability of oils and fats is placed at 10 kg as against the world average of 17 kg. Thus there is an urgent need to step up oilseeds production on a sustainable basis. However with limited chances of area expansion and with emphasis on increasing production, it is essential to exploit the emerging technologies. New developments in biotechnology will make possible the development of genotypes that will provide tailor made vegetable oils for the edible and industrial markets.

It is possible to tap the opportunities available through international collaborations in agri-food bio-diversity and participation of the private sector in developing biotechnological capacity. Agriculture extension and linkages between universities and research institutions need to be strengthened to reinforce each other and to  intelligently utilize synergistically the scarce trained personnel resources.   

India is the third largest oilseed producing country in the world, producing 16 per cent of the world's production. With its rich agro-ecological diversity it is ideally suited for cultivation of most l oilseed crops, like groundnut, rapeseed, mustard, sunflower, sesame, soybean, safflower, castor and linseed. India is the world's largest producer of castor, safflower and sesame, second largest producer of groundnut and rapeseed-mustard, third in linseed and cottonseed and fifth in the production of soyabean and sunflower.

In spite of the premier position that the country holds in the global oilseed scenario, the actual productivity of oilseeds is very low. Indian oilseed scenario has undergone a dramatic change during the last decade with a series of farmer oriented programmes launched by the Technology Mission on Oilseeds along with better availability of crop production technologies, inputs, services, support price policy and restricted imports. Though there has been an area expansion from 19.02 to 26.81 million hectares and increase in productivity from 570 to 931 kg/ha, the oilseeds sector has become a net importer in 1998-99 and 1999-2000 with a huge import of 4.4 and 4.89 million tonnes of edible oils, respectively.

Improving agricultural productivity
Concerted efforts in conventional breeding programmes led to the development of a number of improved cultivators, but the yield levels have been virtually static owing to their susceptibility to biotic and abiotic stresses, and limited genetic variability in the cultivar germplasm. Biotechnology is one of the powerful tools to fight this susceptibility. Some of the objectives of biotechnological improvement of oil crops are increased productivity, modification of plant architecture, improvement of resistance to biotic and abiotic stresses and adaptation to new environments. Additional objectives are concerned with the bio-engineered modification of their fatty acid and protein composition, in order to adapt them to specific food, feed or industrial utilisations. The focus of biotechnological techniques in oilseed crops are directed towards the following aspects:

Acceleration of the conventional plant breeding processes
Modern biotechnology enables transfer of genes between non-related species, precise regulation of gene expression, regeneration of genetically modified plants and reproduction of new plants. Among the annual yielding edible oils, the Brassica spp, soyabean and sunflower have been well studied. In sunflower and Brassica, the techniques of embryo, ovule and ovary culture have been exploited to assist the use of wild germplasm as a source of traits such as disease resistance, drought tolerance and oil quality. Groundnut and sesame crops have been difficult to manipulate in vitro, while reproducible protocols of regeneration are reported in safflower. Among the two important annual non-edible oilseeds, linseed has proved to be amenable to in vitro regenerability, genetic variation and transformation while, castor has proved to be highly recalcitrant in vitro

Genetic modification of the agronomic characteristics
The major oil crops have been genetically modified with emphasis on agronomic traits. At present biotechnological applications are directed to only rapeseed, soyabean and sunflower and there is need to extend this technology to other oilseed crops grown predominantly in India. Castor, cultivated for its industrial oil, is susceptible to a multitude of insect pests and diseases like wilt and Botrytis grey rot hereby, limiting its production. As the genetic variability for these biotic stresses in this monotypic genus is limited, there is a need to incorporate additional variability through gene transfer techniques. Similarly, in case of sunflower and safflower, where insect pests pose a serious threat, transgenics may be developed. Development of transgenics over expressing plants or bacterial derived enzymes like chitinases, glucanases, lectins and others for conferring protection against fungal and bacterial pathogens should be attempted.

Since most of the oilseeds are grown in marginal lands and under rainfed conditions, the engineering of traits that confer resistance to environmental stress, such as drought, frost, water logging or salinity could contribute to increased productivity enhancement

Genetic modification of the fatty acid composition
Biotechnological methods have resulted in the alternation of the type and the proportion of fatty acids specific to individual oil crop varieties. Achievements in this field include the development of enhanced germplasm of rapeseed, soyabean, sunflower, flax, cotton and new industrial oil crops. Particularly significant commercial results have been obtained in the bioengineering of rapeseed, in order to secure and increase the food and non-food consumption of rapeseed oil. The edible oil industry desires oil with a long shelf life and one that can be hardened into margarine and shortenings with little or no nutritionally undesirable trans fatty acids. To meet this market demand, canola oil with reduced levels of linolenic and/or linoleic acids together with concomitant increase in oleic acid are being developed.

The timing of the commercial application of biotechnology in breeding will differ between individual oil crops. At present, the genetic engineering of rapeseed is routine, and genetically modified soyabeans are broadly used. Since the manipulation of genes responsible for fatty acid composition and yields appear to give rise to the same type of modifications in other oilseeds, it can be expected that in the next five years or so other annual oil crops could be genetically modified.....

...CONTD

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